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2020Open access

Uptake and toxicity of clothianidin to monarch butterflies from milkweed consumption

Bargar TA, Hladik ML, Daniels JC. PeerJ, March 2020.

Read the paper Free to read · doi:10.7717/peerj.8669

What this paper is about

Monarch larvae were fed milkweed contaminated with clothianidin from hatching to pupation, and survival, growth and pupation were recorded.

Most neonicotinoid research has concentrated on bees. Land managers restoring monarch habitat near treated fields needed numbers for monarchs specifically.

Summarised here from the abstract below. Not the authors’ words.

Abstract

Recent concern for the adverse effects from neonicotinoid insecticides has centered on risk for insect pollinators in general and bees specifically. However, natural resource managers are also concerned about the risk of neonicotinoids to conservation efforts for the monarch butterfly (Danaus plexippus) and need additional data to help estimate risk for wild monarch butterflies exposed to those insecticides. In the present study, monarch butterfly larvae were exposed in the laboratory to clothianidin via contaminated milkweed plants from hatch until pupation, and the effects upon larval survival, larval growth, pupation success, and adult size were measured. Soils dosed with a granular insecticide product led to mean clothianidin concentrations of 10.8-2,193 ng/g in milkweed leaves and 5.8-58.0 ng/g in larvae. Treatment of soils also led to clothianidin concentrations of 2.6-5.1 ng/g in adult butterflies indicating potential for transfer of systemic insecticides from the soil through plants and larvae to adult butterflies. Estimated LC50s for total mortality (combined mortality of larvae and pupae) and EC50 for larval growth were variable but higher than the majority of concentrations reported in the literature for clothianidin contamination of leaves.

Abstract reproduced from the published paper, which is licensed CC0. Copyright remains with the authors.

Key terms

ToxicityNeonicotinoidMonarch ButterflyClothianidinSwamp Milkweed

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APA Milkweed USA. (2026). Uptake and toxicity of clothianidin to monarch butterflies from milkweed consumption (listing). Retrieved DATE, from https://milkweedusa.com/research/uptake-and-toxicity-of-clothianidin-to-monarch-butterflies-from-2020/
MLA "Uptake and toxicity of clothianidin to monarch butterflies from milkweed consumption (listing)." Milkweed USA, 2026, https://milkweedusa.com/research/uptake-and-toxicity-of-clothianidin-to-monarch-butterflies-from-2020/. Accessed DATE.
Chicago Milkweed USA. "Uptake and toxicity of clothianidin to monarch butterflies from milkweed consumption (listing)." Last modified 2026-08-01. Accessed DATE. https://milkweedusa.com/research/uptake-and-toxicity-of-clothianidin-to-monarch-butterflies-from-2020/.
BibTeX @misc{milkweedusa_researchuptakeandtoxicityofc, title = {Uptake and toxicity of clothianidin to monarch butterflies from milkweed consumption (listing)}, author = {{Milkweed USA}}, year = {2026}, url = {https://milkweedusa.com/research/uptake-and-toxicity-of-clothianidin-to-monarch-butterflies-from-2020/}, note = {Accessed DATE} }